Updated: November 9, 2026

What is produced during transpiration is not a new food or a new growth tissue. The main result is water vapor leaving the plant through tiny openings called stomata, and that water loss drives the movement of water and minerals from the roots upward. If you are trying to understand plant biology, the key idea is simple, transpiration is a process that releases water vapor and creates the pull that keeps the plant supplied.

What transpiration produces

Transpiration produces water vapor at the leaf surface. Inside the leaf, liquid water evaporates from moist cell walls into the air spaces, then diffuses out through the stomata. That is the direct product of the process. The plant does not manufacture a special compound called transpiration, it loses water as vapor and changes internal water pressure as a result.

The second important outcome is not a substance, but a physical force. As water leaves the leaf, it creates transpiration pull, which helps move more water upward through the xylem. This pull carries dissolved minerals from the roots to stems and leaves, supporting growth, cooling, and normal cell function.

What is formed during transpiration

The most direct thing formed during transpiration is a gradient between moist leaf air spaces and the drier outside air. That gradient pushes water vapor out of the stomata. In plant science terms, the process forms a pressure difference that draws water through the plant body.

At the tissue level, transpiration also contributes to a continuous column of water in the xylem. This water column stays under tension while water evaporates from the leaves. That tension is what allows roots to keep absorbing water from the soil and move it upward against gravity.

What plays a role in transpiration

Several structures control transpiration, but the main players are stomata, guard cells, the cuticle, and the xylem. Stomata are pores on the leaf surface. Guard cells open and close those pores. The cuticle is the waxy outer layer that slows water loss. Xylem is the water conducting tissue that supplies water to the leaf.

Stomata and guard cells

Stomata are the main exit points for water vapor. Guard cells change shape to open or close each pore in response to light, carbon dioxide, water status, and internal signals. When stomata open for gas exchange, water vapor escapes at the same time. This is why transpiration and photosynthesis are linked.

Cuticle and leaf surface

The cuticle limits direct evaporation from the leaf surface. A thick cuticle slows water loss, while a thin cuticle allows faster loss. Plants in hot, dry, or windy sites often have thicker cuticles or smaller leaves because those traits reduce transpiration. Leaf hairs, rolled leaves, and sunken stomata also reduce water loss.

Xylem and root uptake

Xylem vessels and tracheids carry water upward from the roots. Transpiration creates the pull that keeps this flow moving. Without that pull, water movement slows and the plant cannot replace water lost from leaves quickly enough. Root hair cells absorb water from soil, then xylem distributes it through the plant.

Molecules involved in transpiration

The main molecule involved in transpiration is water in both liquid and vapor form. Water molecules evaporate from moist cell walls inside the leaf, then move out through stomata. Hydrogen bonding between water molecules helps maintain the continuous water column in xylem, which is essential for upward transport.

Carbon dioxide and oxygen are involved because stomata serve gas exchange, but they are not the main products of transpiration. Carbon dioxide enters the leaf for photosynthesis, and oxygen exits as a byproduct of photosynthesis and respiration. Water vapor is the molecule produced by transpiration itself. Mineral ions such as nitrate, potassium, and calcium are carried in the transpiration stream, but they are transported, not produced.

Factors that change the rate of transpiration

Transpiration rate changes when the air, leaf, or soil changes. Light opens stomata, raising transpiration. Higher temperature speeds evaporation from leaf surfaces. Wind removes humid air around the leaf, keeping the vapor gradient steep. Low humidity also increases water loss because dry air pulls more water vapor from the leaf.

Soil water supply matters too. If the soil is dry, roots absorb less water and guard cells close stomata to reduce loss. Leaf size, stomatal density, and plant species also affect rate. A plant with many stomata and broad leaves loses more water than a plant with fewer stomata and narrow, waxy leaves.

A simple experiment to see transpiration

You can observe transpiration by covering a leafy stem with a clear plastic bag. Use a healthy potted plant or a cut stem with several leaves. Tie the bag around the stem and leave it in bright indoor light or outside in shade for 1 to 2 hours. Water vapor released by the leaves condenses as droplets inside the bag.

  1. Select a leafy branch with intact leaves.
  2. Dry the outside of the leaves so you do not trap surface water.
  3. Place a clear plastic bag over the leaves.
  4. Seal the bag at the stem with a twist tie or tape.
  5. Leave the plant in a warm, bright location for 1 to 2 hours.
  6. Look for condensation on the inside of the bag.

The droplets show that water vapor was produced and released by the plant. If you repeat the test with one plant in moving air and another in still air, the bag in moving air develops condensation faster because wind increases transpiration.

Frequently Asked Questions

What is produced during transpiration in plants?

Water vapor is produced. The plant loses water from its leaves through stomata, and that vapor exits into the air. The process also creates transpiration pull, which moves water and dissolved minerals through the xylem.

What is formed during transpiration?

A water potential gradient forms between the moist interior of the leaf and the drier outside air. That gradient creates tension in the xylem and keeps water moving upward from the roots.

What plays a role in transpiration?

Stomata, guard cells, the cuticle, xylem, light, temperature, humidity, wind, and soil moisture all play a role. Together they control how fast water moves out of the leaf and how quickly the plant can replace it.

Which molecules are involved in transpiration?

Water molecules are the key molecules. Water evaporates from leaf cells and exits as vapor. Carbon dioxide and oxygen are involved in leaf gas exchange, while mineral ions move with the water stream through the plant.